Effective pretreatment of lignocellulosic co-substrates using barley straw-adapted microbial consortia to enhanced biomethanation by anaerobic digestion

Microbial pretreatments have been identified as a compatible and sustainable process with anaerobic digestion compared to energy-intensive physicochemical pretreatments. In this study, barley straw hay co-substrate was pretreated microaerobic straw-adapted microbial (BSAM) consortium prior digestion. The improved digestibility investigated through 16S rRNA gene sequencing, counts C:N ratios. BSAM pretreatment resulted in 15.2 L kg?1 TS of methane yield after 35 days, almost 40 times more than the control. content total biogas produced were 58% (v/v) 10% control, respectively. This research demonstrated that BSAM-based significantly increased surface area lignocellulosic material considerably enhanced biomethanation. study generates new potential bio-research opportunities emerging field digestion-biorefineries.